Part:BBa_K277079

3L.3_23.C2.09

3L.3_23.C2.09 is 374 bases long and is cloned into the pGem-T vector.

3L.3_23.C2.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C2.09 is a constituent of 3L.3_23.C2 (along with 3L.3_23.C2.01, 3L.3_23.C2.02, 3L.3_23.C2.03, 3L.3_23.C2.04, 3L.3_23.C2.05, 3L.3_23.C2.06, 3L.3_23.C2.07, and 3L.3_23.C2.08.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

mutation_affecting_coding_sequence YCL030C_re_remove_BlpI (51..59) removal of BlpI

Sequence (corresponds to coordinates 57875..58248 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 348
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 348
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 348
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 348
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 348
  Illegal AgeI site found at 6
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 369